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Table 1 Kinetic parameters determined for KdgDAb, KdgDCt and KdgDPn at 37 °C using the pH-shift assay with 2.5 mM KPi buffer (pH 7.0)

From: Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases

Enzyme

K m [mM]

k cat [1/s]

k cat/K m [1/mM s]

vmax [U/mg]

KdgDAb

1.0 ± 0.1

4.5 ± 0.02

4.5

8.0 ± 0.27

KdgDCt

1.1 ± 0.1

3.1 ± 0.02

2.8

5.3 ± 0.11

KdgDPn

1.1 ± 0.1

1.7 ± 0.01

1.5

3.0 ± 0.06

KdgDAta

0.5

8.83 ± 0.16

17.0

 

KdgDAbb

0.2

3.9 ± 0.04

19.2

 
  1. a Assay conditions: 50 mM NaPi pH 7.5, 100 mM NaCl, 4 mM NADP+, 22 °C [23]
  2. b Assay conditions: 50 mM Hepes/NaOH pH 7.5, 100 mM NaCl, 5 mM MgCl2, 4 mM NADP+, 10 % glycerol, 22 °C [15]