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Table 1 Kinetic parameters determined for KdgDAb, KdgDCt and KdgDPn at 37 °C using the pH-shift assay with 2.5 mM KPi buffer (pH 7.0)

From: Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases

Enzyme K m [mM] k cat [1/s] k cat/K m [1/mM s] vmax [U/mg]
KdgDAb 1.0 ± 0.1 4.5 ± 0.02 4.5 8.0 ± 0.27
KdgDCt 1.1 ± 0.1 3.1 ± 0.02 2.8 5.3 ± 0.11
KdgDPn 1.1 ± 0.1 1.7 ± 0.01 1.5 3.0 ± 0.06
KdgDAta 0.5 8.83 ± 0.16 17.0  
KdgDAbb 0.2 3.9 ± 0.04 19.2  
  1. a Assay conditions: 50 mM NaPi pH 7.5, 100 mM NaCl, 4 mM NADP+, 22 °C [23]
  2. b Assay conditions: 50 mM Hepes/NaOH pH 7.5, 100 mM NaCl, 5 mM MgCl2, 4 mM NADP+, 10 % glycerol, 22 °C [15]
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BMC Biotechnology

ISSN: 1472-6750

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