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Fig. 5 | BMC Biotechnology

Fig. 5

From: Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases

Fig. 5

Temperature stability. a Incubation of KdgDAb1 (100 % = 6.22 U/mg), KdgDAb2 (100 % = 6.56 U/mg), KdgDCt (100 % = 5.5 U/mg) and KdgDPn (100 % = 3.7 U/mg) at 37 °C; b Incubation of KdgDAb1 (100 % = 8.22 U/mg), KdgDAb2 (100 % = 8.1 U/mg), KdgDCt (100 % = 8.4 U/mg) and KdgDPn (100 % = 5.1 U/mg) at 65 °C; assay: volume 200 μl, 2.5 mM KPi (pH 7.0), 2 mM MgCl2, 25 μg/ml BTB and 5 mM 5-keto-4-D-deoxyglucarate; enzyme was incubated in 100 μl of 5 mM KPi (pH 7.0); only KdgDAb1 differed, where additional 0.5 mM NH4HCO3 was used for enzyme stabilisation

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BMC Biotechnology

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