Fig. 2

Sequence alignment. Multiple sequence alignment of known 5-keto-4-deoxy-D-glucarate dehydratases/decarboxylases using clustal omega [46]. The secondary structures are shown above with thick bars representing α-helices and arrows representing β-sheets. Coloured residues represent conserved residues of the active centre involved in the specific substrate recognition. The lysine residue (light grey) forms the intermediate Schiff base with the substrate. The sequence identity for all three dehydratases/decarboxylases obtained by referring to KdgDAt as the standard and performing pairwise alignment with EMBOSS Needle [40] are as follows: KdgDAb = 47 %, KdgDCt = 49 %, KdgDPn = 52 %