Replacement of carbohydrate binding modules improves acetyl xylan esterase activity and its synergistic hydrolysis of different substrates with xylanase

Table 4 The hydrolysis products of different substrates after 24 h hydrolysis by rAXE1s and XynII

		dC+ X	1+ X	4-2+ X	6+ X	22-2+ X	X
Destarched wheat bran	X1	11.3 ± 1.2	12.1 ± 1.9	12.8 ± 0.5	13.0 ± 1.2	12.9 ± 0.9	8.9 ± 0.7
	X2	18.5 ± 3.1	18.3 ± 2.0	17.8 ± 1.1	18.5 ± 1.1	18.4 ± 1.0	19.1 ± 0.9
	X3	0.2 ± 0.0	0.2 ± 0.0	0.2 ± 0.1	0.2 ± 0.0	0.2 ± 0.1	0.3 ± 0.0
	Total	30.0 ± 2.1	30.6 ± 1.9	30.8 ± 1.3	31.7 ± 1.1	31.5 ± 1.0	28.3 ± 0.9
NaClO₂-treated wheat straw	X1	6.8 ± 1.3	7.4 ± 1.5	7.3 ± 0.9	7.9 ± 0.5	7.5 ± 1.8	6.0 ± 0.3
	X2	15.9 ± 2.4	15.5 ± 1.7	16.4 ± 2.1	16.8 ± 1.7	16.8 ± 1.7	14.6 ± 1.8
	X3	0.2 ± 0.0	0.2 ± 0.1	0.2 ± 0.1	0.2 ± 0.0	0.2 ± 0.0	0.6 ± 0.1
	Total	22.9 ± 1.6	23.1 ± 1.5	23.9 ± 1.6	24.9 ± 1.3	24.5 ± 1.7	21.2 ± 1.3
InAX	X1	8.2 ± 0.7	9.5 ± 0.9	10.1 ± 1.1	11.9 ± 1.2	10.0 ± 1.2	8.0 ± 1.1
	X2	25.5 ± 2.3	25.9 ± 1.8	25.9 ± 1.3	27.2 ± 1.5	29.9 ± 2.6	22.8 ± 2.2
	X3	0.2 ± 0.0	0.2 ± 0.0	0.2 ± 0.0	0.2 ± 0.0	0.2 ± 0.0	0.3 ± 0.0
	Total	33.9 ± 1.1	35.6 ± 1.4	36.2 ± 1.2	39.3 ± 1.4	40.1 ± 1.7	31.1 ± 1.4

The results are expressed as % of xylan (as xylose) in destarched wheat bran, NaClO₂-treated wheat straw or water-insoluble wheat arabinoxylan (inAX). dC AXE1dC, 1 AXE1; 4-2 AXE1dC-CBM4-2, 6 AXE1dC-CBM6 22-2 AXE1dC-CBM22-2; X XynII

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