Table 1 Specific activities and kinetic parameters of rAXE1s towards 4-methylumbelliferyl acetate
|  | Specific activity (IU/μmol) | K m (μmol/L) | V max (IU/μmol) | K cat (s−1) | K cat/K m (L · μmol−1 s−1) |
|---|---|---|---|---|---|
AXE1dC | 26,061 ± 469 | 86.79 ± 9.59 | 29,122 ± 676 | 485.4 ± 11.3 | 5.59 ± 1.17 |
AXE1 | 23,517 ± 177 | 86.76 ± 9.46 | 27,350 ± 626 | 455.8 ± 10.4 | 5.25 ± 1.09 |
AXE1dC-CBM4-2 | 45,708 ± 939 | 98.69 ± 14.15 | 53,842 ± 1718 | 897.4 ± 28.6 | 9.09 ± 2.02 |
AXE1dC-CBM6 | 22,652 ± 739 | 78.50 ± 9.85 | 29,080 ± 732 | 484.7 ± 12.2 | 6.17 ± 1.24 |
AXE1dC-CBM22-2 | 26,248 ± 550 | 94.04 ± 7.12 | 33,706 ± 504 | 561.8 ± 8.4 | 5.97 ± 1.17 |