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Table 1 Specific activities and kinetic parameters of rAXE1s towards 4-methylumbelliferyl acetate

From: Replacement of carbohydrate binding modules improves acetyl xylan esterase activity and its synergistic hydrolysis of different substrates with xylanase

  Specific activity (IU/μmol) K m (μmol/L) V max (IU/μmol) K cat (s−1) K cat/K m (L · μmol−1 s−1)
AXE1dC 26,061 ± 469 86.79 ± 9.59 29,122 ± 676 485.4 ± 11.3 5.59 ± 1.17
AXE1 23,517 ± 177 86.76 ± 9.46 27,350 ± 626 455.8 ± 10.4 5.25 ± 1.09
AXE1dC-CBM4-2 45,708 ± 939 98.69 ± 14.15 53,842 ± 1718 897.4 ± 28.6 9.09 ± 2.02
AXE1dC-CBM6 22,652 ± 739 78.50 ± 9.85 29,080 ± 732 484.7 ± 12.2 6.17 ± 1.24
AXE1dC-CBM22-2 26,248 ± 550 94.04 ± 7.12 33,706 ± 504 561.8 ± 8.4 5.97 ± 1.17