From: Event-specific detection of transgenic potato AV43-6-G7 using real-time and digital PCR methods
Prepared concentration | AV43-6-G7 potato genome DNA | Total template DNA | Copies of AV43-6-G7 potato genome | Group average concentration | Group RSD |
---|---|---|---|---|---|
100 % | 50 ng/ reaction | 50 ng/ reaction | 57948.5 | 100.2 % | 0.5 % |
50 % | 25 ng/ reaction | 50 ng/ reaction | 28974.5 | 53.4 % | 0.2 % |
10 % | 5 ng/ reaction | 50 ng/ reaction | 5795.0 | 10.6 % | 2.8 % |
1 % | 0.5 ng/ reaction | 50 ng/ reaction | 579.5 | 1.1 % | 4.0 % |
0.1 % | 0.05 ng/ reaction | 50 ng/ reaction | 58.0 | 0.12 % | 8.2 % |