From: Event-specific detection of transgenic potato AV43-6-G7 using real-time and digital PCR methods
Name | Sequence and labled fluorescence (5′- -3′) | Origin |
---|---|---|
potato endogenous gene fru -F | CTGCCTCCGTCAAGATTTGGTCACT | [12] |
potato endogenous gene fru-R | CTCTTCCCTTTCTTGATGG | [12] |
potato endogenous gene fru-P | FAM-ACTTGTAATTCATCAAGCCAT- BHQ1 | [12] |
MAV43 R1 | GCAAGCTTGGCGTAATCATGGTCATA | this study |
MAV43-R 2 | CGCTCACAATTCCACACAACATACGA | this study |
AP1 primer | GTAATACGACTCACTATAGGGC | this study |
AP 2 primer | ACTATAGGGCACGCGTGGT | this study |
Potato AV43-6-G7 primerF | GGTATCAGGTTCTGGAATAAGACCAA | this study |
Potato AV43-6-G7 primerR | TGTCGTGCCAGCTGCATTA | this study |
Potato AV43-6-G7 Probe | FAM-CCCGCGCGTTGGCCGAT-BHQ1 | this study |
Potato AV43-6-G7 Probe used in duplex ddPCR | VIC-CCCGCGCGTTGGCCGAT-BHQ1 | this study |