Skip to main content

Table 1 Primers and probe in this study

From: Event-specific detection of transgenic potato AV43-6-G7 using real-time and digital PCR methods

Name

Sequence and labled fluorescence (5′- -3′)

Origin

potato endogenous gene fru -F

CTGCCTCCGTCAAGATTTGGTCACT

[12]

potato endogenous gene fru-R

CTCTTCCCTTTCTTGATGG

[12]

potato endogenous gene fru-P

FAM-ACTTGTAATTCATCAAGCCAT- BHQ1

[12]

MAV43 R1

GCAAGCTTGGCGTAATCATGGTCATA

this study

MAV43-R 2

CGCTCACAATTCCACACAACATACGA

this study

AP1 primer

GTAATACGACTCACTATAGGGC

this study

AP 2 primer

ACTATAGGGCACGCGTGGT

this study

Potato AV43-6-G7 primerF

GGTATCAGGTTCTGGAATAAGACCAA

this study

Potato AV43-6-G7 primerR

TGTCGTGCCAGCTGCATTA

this study

Potato AV43-6-G7 Probe

FAM-CCCGCGCGTTGGCCGAT-BHQ1

this study

Potato AV43-6-G7 Probe used in duplex ddPCR

VIC-CCCGCGCGTTGGCCGAT-BHQ1

this study