Table 4 Comparison of several reported biocatalysts for producing (R)-CHBE
Enzyme | SsAR | SsALR | CpSADH | BYueD | Gox2036 | LEK | BgADH3 |
|---|---|---|---|---|---|---|---|
Family | AKR | AKR | MDR | - | SDR | AKR | SDR |
Solvent system | water/n-butyl acetate | water | water | water/toluene | water | water | water/octanol |
Cofactor enzyme | GDH | GDH | / | GDH | GDH | GDH | GDH |
Cosubstrate | G | G | IPA | G | G | G | G |
External cofactor | NADP+ | NADP+ | Without | NADP+ | NAD(H) | Without | NADP+ |
Substrate loading (mmol) | 91 | 0.61 | 1.525 | 13 | 0.06 | 111 | 1200 |
Biocatalyst (g) | 2 (wet) | 0.4 (wet) | / | 0.5 (dry) | 0.1 (wet) | 17 (dry) | 8 (dry) |
Reaction volume (mL) | 50 | 10 | 25 | 20 | 10 | / | 1000 |
Reaction time (h) | 50 | 10 | 17 | 5 | 24 | 1 | 20 |
ee of (R)-CHBE | 91.7 | 99 | 99 | 99.6 | 100 | >99 | 99.9 |
References | [15] | [48] | [49] | [18] | [14] | [17] | This work |