Fig. 6

Production of (R)-CHEB using co-expression E. coli cells in a water/octanol biphasic system with a substrate fed-strategy. Reduction of COBE using sonicated co-expressed E. coli cells (8 g DCW) was conducted at 30 °C in 0.5 L phosphate buffer (pH 6.5) containing glucose (1800 mmol) and COBE (600 mmol) and 0.5 L octanol. COBE (300 mmol) together with 3 equiv. glucose were added to the system at 6 h and 12 h, respectively