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Fig. 4 | BMC Biotechnology

Fig. 4

From: Efficient biosynthesis of ethyl (R)-4-chloro-3-hydroxybutyrate using a stereoselective carbonyl reductase from Burkholderia gladioli

Fig. 4

Schematic presentation of the co-expression plasmid contained BgADH3 and GDH genes and SDS-PAGE analysis. a Structure of the co-expression plasmid pCDFDuet-1-BgADH3-GDH. b SDS-PAGE analysis of recombinant co-expressed protein. Lane 1, protein molecular weight marker; Lane 2, soluble fraction of E. coli/pET28b-BgADH3; Lane 3, precipitate of E. coli/pET28b-BgADH3; Lane 4, soluble fraction of E. coli/pET28b-GDH; Lane 5, precipitate of E. coli/pET28b-GDH; Lane 6, soluble fraction of E. coli/pCDFDuet-1-BgADH3-GDH; Lane 7, precipitate of E. coli/pCDFDuet-1-BgADH3-GDH; Lane 8, E. coli/pCDFDuet-1-BgADH3-GDH without induction

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BMC Biotechnology

ISSN: 1472-6750

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