Table 1 Primers used in present study
Gene | Application type | Primer set | Forwad (5’-3’) | Reverse (5’-3’) | Length of target fragment (bp) |
|---|---|---|---|---|---|
TcTre1-1 | cDNA cloning | TcTre1-1 | GAACCACTTGTCCCTTGTC | TGCTAATCAAGTGGGCTT | 1626 |
qRT-PCR | QTcTre1-1 | AACGACTCGCAATGGCTGG | CGGAGGCGTAGTGGAATAGAG | 127 | |
dsRNA synthesis | dsTcTre1-1 | CGACCTGAAATTAGCCCAGAAG | GGCTCCCCCACTCTTTC | 357 | |
TcTre1-2 | cDNA cloning | TcTre1-2 | CGTTTCATGTCGTTGTG | GAACACCACAGAGACGTAA | 1692 |
qRT-PCR | QTcTre1-2 | GTGCCCAATGGGTTTATCG | CAACCACAACACTTCCTTCG | 261 | |
dsRNA synthesis | dsTcTre1-2 | GGACAATGAGTTGGGGCAG | AACCGCGTAAAATCGCTCG | 493 | |
TcTre1-3 | cDNA cloning | TcTre1-3 | ATGCCTTTCCTCCTACTTCTCACCC | GGTACTACGGCCGGGAAAAATAA | 1647 |
qRT-PCR | QTcTre1-3 | CCTCTCATTCGTCACAAGCG | AAGCGTTTGATTTCTTTGCG | 205 | |
dsRNA synthesis | dsTcTre1-3 | GGGACCGTGGGTTAAACCAAC | CTGTGGCCGCTGAACTGAA | 376 | |
TcTre1-4 | cDNA cloning | TcTre1-4 | ATGAAGTCTCTCCTCGTTTCG | GATTTTACGCGGTTTCGG | 1662 |
qRT-PCR | QTcTre1-4 | ACGGTGCCCGCATCTACTA | GTGTAGGTGGTCCCGTTCTTG | 187 | |
dsRNA synthesis | dsTcTre1-4 | ACGATATCGAGCTCAGAGTCC | TACGTCTCTGTGGTGTTCGC | 462 | |
TcTre2 | cDNA cloning | TcTre2 | TTAGTGTGTTGTGCGTTTGT | TGCGAAGAGCACGAGATAA | 1929 |
qRT-PCR | QTcTre2 | CTCAGCCTGGCCCTTAGTTG | GGAGTCCTCGTAGATGCGTT | 120 | |
dsRNA synthesis | dsTcTre2 | GGTGCGCTCCAACTACAAAG | GCTCCGCAGTTCCGTGTAG | 399 | |
TcTPS | qRT-PCR | QTcTPS | CCTTGTTCCACTCAATGCCCGAC | GTCATGTATCCAGATCAAGGGAAC | 105 |
TcHk1 | Â | QTcHk1 | CGCACCGAATGCCAGAATC | GACCCACCCGACATCGATT | 141 |
TcHk2 | Â | QTcHk2 | CGAATCGGCCTAATAGTTGGC | GACGGAGCCCTCGATTTCAT | 155 |
TcCHS1a | Â | QTcCHS1a | CGTATAGCCGCCGACTTGAA | CCTATGACGAGAGCACCCAAGA | 173 |
TcCHS1b | Â | QTcCHS1b | CCAGGATTGCAAAGGAGTTG | TTGAGGAAACGTCCGAGGTC | 179 |
TcCHS2 | Â | QTcCHS2 | GAGTTGTGGCAATGTTCTCGC | GTGGTGTGGCCCTTGGTT | 116 |
TcGS | Â | QTcGS | GGAGTTTCATGCACGGACTG | GTTGGCTTGTCGATGGGAA | 105 |
TcGP1 | Â | QTcGP1 | GCGAGCGACTATGAACTGATG | TTGCGTGTCGGAAACTACAT | 104 |
TcGP2 | Â | QTcGP2 | GGACGAATATTACACAAGTTACGAC | CAGACCCACGATTACGCTT | 119 |
TcGPI | Â | QTcGPI | GTGATGCCGGAGGTGAAT | CACGTCGGTGATGGGCTT | 112 |
TcGFAT | Â | QTcGFAT | GGAACTGGACATGGACCGTA | GAACGGTGAGGATGCGAGTT | 126 |
TcPFK | Â | QTcPFK | GAGCAAGGACATGGAAGGGAA | CCAACCCAACCAGCCACTT | 158 |
Tcβ-actin |  | QTcactin | AGGGCGTCATGGTCGGTAT | TTCTACAACGAGCTCCGCG | 165 |
GFP | dsRNA synthesis | dsGFP | AAGGGCGAGGAGCTGTTCACCG | CAGCAGGACCATGTGATCGCGC | 206 |