Fig. 2From: Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum Changes in the mRNA levels of the five TcTres after specific RNAi in penultimate-instar larvae. The penultimate-instar larvae were selected as the targets for dsRNA injection. a, b, c, d and e represent the mRNA levels of TcTre1-1, TcTre1-2, TcTre1-3, TcTre1-4 and TcTre2 relative to Tcβ-actin mRNA level after RNAi targeting all five of the TcTres, analysed via qRT-PCRBack to article page