Fig. 1From: Knockdown of five trehalase genes using RNA interference regulates the gene expression of the chitin biosynthesis pathway in Tribolium castaneum Analysis of the five TcTre transcripts in different tissues of last-instar larvae using semi-quantitative PCR (RT-PCR). Total RNA was extracted from various tissues: fat body (Fb), midgut (Mg), cuticle (Cu) and Malpighian tubules (Mt). The expression of Tcβ-actin in the four tissues was used to standardize the relative expression levels of the five TcTre genes. CK represents PCR products without a template used as the controlsBack to article page