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Fig. 5 | BMC Biotechnology

Fig. 5

From: Design, purification and assessment of GRP78 binding peptide-linked Subunit A of Subtilase cytotoxic for targeting cancer cells

Fig. 5

The cleavage of GRP78 and inhibition of tumor cells by GBP-SubA. a. The total cellular lysate was extracted from HL-7702, DLD1 and HepG2 cells that were treated with different concentrations of GBP-SubA for 24 h, and the cleavage of GRP78 was analyzed by western blotting. b. Cell inhibition ratios of GBP-SubA were determined by the MTT assay. HL-7702, DLD1 and HepG2 cells were incubated with different concentrations of GBP-SubA for 48 h. *p < 0.05, **p < 0.01, compared with the control. c. The cellular inhibition of GBP-SubA was determined by cytometry. HL-7702, DLD1 and HepG2 cells were incubated with 0.1 μg/mL GBP-SubA for 48 h. *p < 0.05, **p < 0.01, compared with the control

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