Fig. 4

Detection of cell surface GRP78. a. The surface-GRP78 of HL-7702, DLD1 and HepG2 cells were stained by anti-GRP78 antibody and analyzed by flow cytometry, and the HL-7702 stained with IgG served as the negative control. b. The mean fluorescence intensity of HL-7702, DLD1 and HepG2 cells stained with anti-GRP78 antibody and the negative control stained with IgG. c. Cell membrane proteins were extracted from HL-7702, DLD1 and HepG2 cells. Equal amount of protein was loaded per lane for western blotting detection of GRP78