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Fig. 1 | BMC Biotechnology

Fig. 1

From: Design, purification and assessment of GRP78 binding peptide-linked Subunit A of Subtilase cytotoxic for targeting cancer cells

Fig. 1

Construction of the recombinant plasmid pET-28a-GBP-SubA. a. Both pET-28a and pMD19-GBP-SubA plasmids were digested by Bam HI and Xho I. Lane 1: pET-28a vector (after digestion); lane 2: pMD19-GBP-SubA plasmid (after digestion). b. GBP-SubA was successfully inserted into pET-28a vector confirmed by colony PCR. Lane 1: PCR products amplified by GBP-SubA primers. c. Both pET-28a and recombinant pET-28a-GBP-SubA were digested by Bam HI or/and Xho I. Lane 1: pET-28a vector (before digestion); lane 2: recombinant pET-28a-GBP-SubA (digested with Bam HI only); lane 3: recombinant pET-28a-GBP-SubA (digested with Bam HI and Xho I); Lane M: marker

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BMC Biotechnology

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