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Table 1 Bacterial strains and plasmids used in this study

From: High-specificity synthesis of novel monomers by remodeled alcohol hydroxylase

Plasmid or strain Relevant genotype or description Reference
Plasmids
 pCOLADuet-1 ColA origin; KanR; PT7 Novagen
 pLQ12 ColA origin; KanR; PT7:: P450 BM3 This study
 pZZ1 ColA origin; KanR; PT7:: P450 BM3J This study
 pV78A ColA origin; KanR; PT7:: P450 BM3V78A This study
 pT175I ColA origin; KanR; PT7:: P450 BM3T175I This study
 pA184V ColA origin; KanR; PT7:: P450 BM3A184V This study
 pF205C ColA origin; KanR; PT7:: P450 BM3F205C This study
 pS226R ColA origin; KanR; PT7:: P450 BM3S226R This study
 pH236Q ColA origin; KanR; PT7:: P450 BM3H236Q This study
 pE252G ColA origin; KanR; PT7:: P450 BM3E252G This study
 pR255S ColA origin; KanR; PT7:: P450 BM3R255S This study
 pA290V ColA origin; KanR; PT7:: P450 BM3A290V This study
 pL353V ColA origin; KanR; PT7:: P450 BM3L353V This study
 pJ2 ColA origin; KanR; PT7:: P450 BM3JA78F This study
 pJ2-R47L ColA origin; KanR; PT7:: P450 BM3JA78FR47L This study
 pJ2-F87A ColA origin; KanR; PT7:: P450 BM3JA78F87A This study
Strains
 BL21(DE3) E. coli B dcm ompT hsdS(rB mB ) gal Invitrogen
 LQ12 BL21(DE3) harboring pLQ12 This study
 ZZ1 BL21(DE3) harboring pZZ1 This study
 V78A BL21(DE3) harboring pV78A This study
 T175I BL21(DE3) harboring pT175I This study
 A184V BL21(DE3) harboring pA184V This study
 F205C BL21(DE3) harboring pF205C This study
 S226R BL21(DE3) harboring pS226R This study
 H236Q BL21(DE3) harboring pH236Q This study
 E252G BL21(DE3) harboring pE252G This study
 R255S BL21(DE3) harboring pR255S This study
 A290V BL21(DE3) harboring pA290V This study
 L353V BL21(DE3) harboring pL353V This study
 J2 BL21(DE3) harboring pJ2 This study
 J2-R47L BL21(DE3) harboring pJ2-R47L This study
 J2-F87A BL21(DE3) harboring pJ2-F87A This study