Table 1 Bacterial strains and plasmids used in this study
From: High-specificity synthesis of novel monomers by remodeled alcohol hydroxylase
Plasmid or strain | Relevant genotype or description | Reference |
|---|---|---|
Plasmids | ||
pCOLADuet-1 | ColA origin; KanR; PT7 | Novagen |
pLQ12 | ColA origin; KanR; PT7:: P450 BM3 | This study |
pZZ1 | ColA origin; KanR; PT7:: P450 BM3J | This study |
pV78A | ColA origin; KanR; PT7:: P450 BM3V78A | This study |
pT175I | ColA origin; KanR; PT7:: P450 BM3T175I | This study |
pA184V | ColA origin; KanR; PT7:: P450 BM3A184V | This study |
pF205C | ColA origin; KanR; PT7:: P450 BM3F205C | This study |
pS226R | ColA origin; KanR; PT7:: P450 BM3S226R | This study |
pH236Q | ColA origin; KanR; PT7:: P450 BM3H236Q | This study |
pE252G | ColA origin; KanR; PT7:: P450 BM3E252G | This study |
pR255S | ColA origin; KanR; PT7:: P450 BM3R255S | This study |
pA290V | ColA origin; KanR; PT7:: P450 BM3A290V | This study |
pL353V | ColA origin; KanR; PT7:: P450 BM3L353V | This study |
pJ2 | ColA origin; KanR; PT7:: P450 BM3JA78F | This study |
pJ2-R47L | ColA origin; KanR; PT7:: P450 BM3JA78FR47L | This study |
pJ2-F87A | ColA origin; KanR; PT7:: P450 BM3JA78F87A | This study |
Strains | ||
BL21(DE3) | E. coli B dcm ompT hsdS(rB −mB −) gal | Invitrogen |
LQ12 | BL21(DE3) harboring pLQ12 | This study |
ZZ1 | BL21(DE3) harboring pZZ1 | This study |
V78A | BL21(DE3) harboring pV78A | This study |
T175I | BL21(DE3) harboring pT175I | This study |
A184V | BL21(DE3) harboring pA184V | This study |
F205C | BL21(DE3) harboring pF205C | This study |
S226R | BL21(DE3) harboring pS226R | This study |
H236Q | BL21(DE3) harboring pH236Q | This study |
E252G | BL21(DE3) harboring pE252G | This study |
R255S | BL21(DE3) harboring pR255S | This study |
A290V | BL21(DE3) harboring pA290V | This study |
L353V | BL21(DE3) harboring pL353V | This study |
J2 | BL21(DE3) harboring pJ2 | This study |
J2-R47L | BL21(DE3) harboring pJ2-R47L | This study |
J2-F87A | BL21(DE3) harboring pJ2-F87A | This study |