Fig. 3
From: High-specificity synthesis of novel monomers by remodeled alcohol hydroxylase
Regiospecificities of the native P450BM3, P450BM3J and corresponding 10 single mutants of P450BM3J. Decanol was used as the substrate, and 1,7-, 1,8- and 1,9-decanediols were analyzed by GC-MS. The ratio of 1,7-decanediol to total decanediols increased from 34.0 % in BM3 to ~50 % in R255S, suggesting the increased ratio of 1,7-decanediol to total decanediols in BM3J is mainly attributed to the substitution of Arg255 to Ser255. The other 9 amino acid substitutions may have a combined effect on the constriction of the substrate-binding channel and the change of the substrate orientation