Fig. 3
From: Improvement of betulinic acid biosynthesis in yeast employing multiple strategies
Comparison of the BA-producing activities between BPLO and the previously reported LOs (CrAO and CYP716A15) in yeast. The LO genes were individually co-expressed with AtLup1 in the WAT11 yeast strain. a Total ion chromatograms were shown for the products by expressing BPLO, CrAO and CYP716A15; b the relative amounts of the products lupeol (LUP), betulin (BN), and betulinic acid (BA) produced by the transgenic yeast cells expressing the individual LO. “LUP/BN + BA + LUP” represents the ratio of the yield of the total products (LUP, BN and BA) divided by the LUP yield. Similarly, “BN/BN + BA + LUP” and “BA/BN + BA + LUP” represent the ratios of the total product yield divided by the yield of BN and BA, respectively. Error bars represent the standard errors of the means calculated from two biological replicates