Fig. 3

Simultaneous sequence editing results of simplex and multiplex editing. a Targeted mutation efficiency of simplex and multiplex edited plants. Editing rate showed the percentage of T0 transgenic lines with stable heritable editing within all T0 lines. b The DNA sequence of the simplex edited genes is provided. The 20-bp gRNA spacer sequence for the Cas9/gRNA complex is in blue, and the PAM site is in red. Deleted nucleotides are depicted as dots, and inserted nucleotides are shown in green. The lengths of the insertions and/or deletions (In/Del) and the frequencies (Freq) are presented. WT, wild type; M1, mutant1. c The DNA sequence of the multiplex edited genes is provided. The 20-bp gRNA spacer sequence for the Cas9/gRNA complex is in blue, and the PAM site is in red. Deleted nucleotides are depicted as dots, and inserted nucleotides are shown in green. The lengths of the insertions and/or deletions (In/Del) and the frequencies (Freq.) are presented. WT, wild type; M1, mutant1; T1, target1. Gel pictures show PCR products amplified from PPR/CAS9-8, 9 and −13 lines. Left lane, WT; right lane, mutant