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Fig. 1 | BMC Biotechnology

Fig. 1

From: High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize

Fig. 1

Simplex and multiplex editing vector construction strategy. a & b Structure of simplex and multiplex editing binary vector based on pCAMBIA3301. P, promoter; T, terminator; Ubi P, ubiquitin promoter; NLS, nuclear localization sequence; Nos T, nopaline synthase terminator; BAR, phosphinothricin R; Kan R, kanamycin resistance gene; LB, left border; RB, right border; TGU, tRNA-gRNA unit

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