Fig. 6
From: Lentiviral expression system for the purification of secreted proteins from human cell cultures
Antiviral effect of purified sCD4. a Binding of sCD4 to gp120. b Inhibition of HIV entry. 5 × 104 TZM-bl cells were infected with replication-incompetent virus pseudotyped with the indicated HIV Env (TCID50 of 5 × 103) in the presence of the indicated concentration of sCD4. On day 2 post-infection, β-galactosidase activity was determined by incubation with X-gal. For each sample, the number of infected cells was determined by counting the blue cells from 3 random microscopy images. c Representative microscopy images of cells infected in the presence (16 μg/ml) or absence of sCD4. The results are representative of 2 independent experiments