Fig. 3
From: Lentiviral expression system for the purification of secreted proteins from human cell cultures
Human 293 T cells can be transduced at high MOIs. 293 T cells were transduced with LV-CMV-AAT-sCD4 at the indicated MOIs. 2 × 105 gene-modified 293 T cells were seeded in a 12-well plate in 1 ml media and cultured for 4 days. a Concentration of sCD4 in the culture supernatants determined by His-tag ELISA. b Cell growth analyzed by microscopy and cell viability determined by MTT-assay. The results are representative of at least 2 independent experiments