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Fig. 1 | BMC Biotechnology

Fig. 1

From: Lentiviral expression system for the purification of secreted proteins from human cell cultures

Fig. 1

Lentiviral transduction of HEK 293 T cells. a Schematic diagram of the sCD4 open reading frame and the bicistronic lentiviral vector used in this study. LTR = long terminal repeat; Ψ = packaging signal; RRE = rev response element; cPPT = central polypurin tract; IRES = internal ribosome entry site; ΔU3 LTR = LTR with a deletion in the U3 promoter/enhancer region. b-d 293 T cells were transduced with LV-CMV-sCD4 at the indicated MOIs. 2 × 105 gene-modified 293 T cells were seeded in a 12-well plate in 1 ml media and grown for 4 days. The cells were trypsinized and analyzed by (b) fluorescence microscopy and (c) flow cytometry. d Supernatants were collected, centrifuged, and analyzed by Western blot using an anti-His-tag antibody. MFI = median fluorescence intensity. The results are representative of at least 2 independent experiments

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