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Fig. 1 | BMC Biotechnology

Fig. 1

From: Protoplast transformation as a potential platform for exploring gene function in Verticillium dahliae

Fig. 1

Optimization of protoplast isolation and regeneration. a Protoplast isolation efficiency from mycelia cultured for 16 to 24 h and then treated for 2.5 h in 10 ml driselase mixture. b Protoplast isolation efficiency after various digestion times with driselase. Mycelia were harvested at 20 h post inoculation. c Regeneration efficiency in different media. Protoplasts (200 μl of 106/ml) were cultured in 5 ml TB3, CM or Czapek-Dox broth. After 18 h, the regeneration efficiency was measured as the number of protoplast regenerated out of total number of protoplast cultured multiplied by 100

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