Table 1 Purification of BCAI expressed in recombinant E. coli
Purification step | Protein(mg) | Total activity(U) | Specific activity(U mg-1) | Yield(%) | Purification fold |
|---|---|---|---|---|---|
Crude extract | 39.4 | 89.9 | 2.3 | 100 | 1 |
Heat treatment | 15.7 | 78.4 | 5.0 | 87.2 | 2.2 |
HisTrap HP 5Â mL column | 2.3 | 18.3 | 8.0 | 20.3 | 3.5 |