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Table 1 Purification of BCAI expressed in recombinant E. coli

From: Characterization of an L-arabinose isomerase from Bacillus coagulans NL01 and its application for D-tagatose production

Purification step Protein(mg) Total activity(U) Specific activity(U mg-1) Yield(%) Purification fold
Crude extract 39.4 89.9 2.3 100 1
Heat treatment 15.7 78.4 5.0 87.2 2.2
HisTrap HP 5 mL column 2.3 18.3 8.0 20.3 3.5
  1. Then enzyme activity was measured using L-arabinose as a substrate