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Table 1 Protein yield at different purification steps

From: Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle

Steps Procedure Protein recovery (%)
1 Ni2+ affinity chromatography to capture SUMO-VP proteins 70 %-80 %
2 Cleavage of SUMO tag using Ulp1 to release the un-tagged VP proteins 60 %-70 %
3 Q Sepharose Fast Flow anion exchange chromatography to obtain the pure VP proteins 50 %-60 %
  Total yield: 20 %-35 %