Table 1 Protein yield at different purification steps
Steps | Procedure | Protein recovery (%) |
|---|---|---|
1 | Ni2+ affinity chromatography to capture SUMO-VP proteins | 70 %-80 % |
2 | Cleavage of SUMO tag using Ulp1 to release the un-tagged VP proteins | 60 %-70 % |
3 | Q Sepharose Fast Flow anion exchange chromatography to obtain the pure VP proteins | 50 %-60 % |
Total yield: | 20 %-35 % |