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Fig. 1 | BMC Biotechnology

Fig. 1

From: Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle

Fig. 1

SDS-PAGE and western-blot analysis of FMDV capsid proteins expressed in E. coli. a SDS-PAGE of FMDV capsid protein expression without purification. M, protein marker; lane 1, total protein without IPTG induction; lane 2, total protein after IPTG induction; lane 3, supernatant protein after IPTG induction; lane 4, pellet protein after IPTG induction; b Western-blot of FMDV capsid protein expression without purification. M, protein marker; lane 1, supernatant protein after IPTG induction; lane 2, pellet protein after IPTG induction; c M, protein marker; lane 1, purified protein complex (1ul load); lane 2, purified protein complex (10ul load); lane 3, purified protein complexes after Ulp 1 protease treatment (1ul load); lane 4, purified protein complexes after Ulp 1 protease treatment (10ul load)

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BMC Biotechnology

ISSN: 1472-6750

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