Table 3 E. coli strains and plasmids used in this study
Name | Characteristics | Source |
|---|---|---|
Strains | ||
 BW25113 | lacI q rrnB T14ΔlacZWJ16 hsdR514ΔaraBAD AH33 | NBRP-E. coli at NIG |
 BW25113(DE3) | lacI q rrnB T14ΔlacZWJ16 hsdR514ΔaraBAD AH33dcm (DE3) | This study |
 BKS1 | BW25113(DE3) harboring pCDF-pck | This study |
 BKS2 | BW25113 harboring pCDF-pck | This study |
 BKS3 | BL21(DE3) harboring pCDF-pck | This study |
 BKS4 | BW25113(DE3) ΔptsG::FRT | This study |
 BKS5 | BKS4 ΔpykA::FRT | This study |
 BKS6 | BKS5 Δppc::FRT | This study |
 BKS7 | BKS6 ΔmaeA::FRT | This study |
 BKS8 | BKS7 ΔmaeB::FRT | This study |
 BKS9 | BKS8 Δsdh::FRT | This study |
 BKS10 | BKS9 ΔiclR::FRT | This study |
 BKS11 | BKS10 harboring pCDF-pck-ecaA | This study |
 BKS12 | BKS8 harboring pCDF-pck | This study |
 BKS13 | BKS8 harboring pCDF-pck and pRSF-anti-pykF | This study |
 BKS14 | BKS8 harboring pCDF-pck and pBldg-anti-pykF | This study |
 BKS15 | BKS11 harboring pBldg-anti-pykF | This study |
Plasmids | ||
 pKD3 | FRT(FLP recognition target) sites; CmR | (Datsenko and Wanner 2000) |
 pKD46 | Red recombinase expression vector; AmpR | (Datsenko and Wanner 2000) |
 pCP20 | FLP expression vector; AmpR,CmR | (Datsenko and Wanner 2000) |
 pETDuet-1 | pBR322 ori with PT7; AmpR | Novagen |
 pCDFDuet-1 | CDF ori with PT7; StrR | Novagen |
 pRSFDuet-1 | RSF ori with PT7; KanR | Novagen |
 pBldgbrick2 | p15A ori with PlacUV5; CmR | (Yao et al, 2013) |
 pCDF-pck | pCDFDute-1 with pck | This study |
 pCDF-pck-ecaA | pCDFDuet-1 with pck and ecaA | This study |
 pRSFM1 | pRSF without RBS sequence | This study |
 pRSF-anti-pykF | pRSFM1 with anti-pykF | This study |
 pBldg-anti-pykF | pBldgbrick2 with anti-pykF | This study |