Targeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli

BMC Biotechnology

Table 2 Parameters of succinate production by engineered E. coli strians during anaerobic fermentation

Strains	Growth rate (h⁻¹)	Titer (mM)			Yield (mol/mol of glucose)			Specific productivity (mmol gDCW⁻¹ h⁻¹)			Productivity (mmol L⁻¹ h⁻¹)
Strains	Growth rate (h⁻¹)	Succinate	Lactate	Acetate	Succinate	Lactate	Acetate	Succinate	Lactate	Acetate	Succinate	Lactate	Acetate
BKS10	0.071 ± 0.002	8.65 ± 0.73	12.06 ± 0.70	27.13 ± 2.56	0.31 ± 0.02	0.43 ± 0.02	0.98 ± 0.09	1.09 ± 0.06	1.47 ± 0.20	3.31 ± 0.13	0.12 ± 0.01	0.17 ± 0.01	0.39 ± 0.04
BKS11	0.052 ± 0.003^**	25.51 ± 1.79^**	7.82 ± 0.63^**	23.52 ± 1.53	0.92 ± 0.06^**	0.28 ± 0.02^**	0.85 ± 0.05	3.96 ± 0.13^**	1.18 ± 0.04^*	3.54 ± 0.08	0.36 ± 0.03^**	0.11 ± 0.01	0.34 ± 0.02
BKS15	0.043 ± 0.002^*	30.12 ± 3.31	6.55 ± 0.33^*	13.22 ± 1.64^**	1.08 ± 0.11	0.24 ± 0.01^*	0.48 ± 0.06^**	5.89 ± 0.41^**	1.20 ± 0.07	2.42 ± 0.19^**	0.43 ± 0.05	0.09 ± 0.01	0.19 ± 0.02^**

The data are shown as mean values ± standard deviation (SD) of three replicates. Asterisks indicate p-values (**p < 0.01, *p < 0.05) in which BKS11 was compared to BKS10 and BKS15 was compared to BKS11

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