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Fig. 1 | BMC Biotechnology

Fig. 1

From: Targeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli

Fig. 1

Targeted engineering of the central carbon metabolism for succinate production in E. coli. Red crosses represent deletion of gene and the reactions affected by the deletion are indicated with grey. The black arrows of the reactions involved in the overexpressed genes are thickened. Red ┫ represents inhibition of pykF expression by anti-pykF sRNA either on high-copy-number plasmid (pRSFDuet-1) (H) or low-copy-number plasmid (pBldgbrick2) (L). Genes coding the corresponding enzymes in the pathways: ptsG, glucose phosphotransferase; pykF, pyruvate kinase I; pykA, pyruvate kinase II; ppc, PEP carboxylase; pck, PEP carboxykinase; ecaA, carbonic anhydrase; iclR, transcriptional repressor IclR; aceA, isocitrate lyase; aceB, malate synthase; aceK, isocitrate dehydrogenase kinase/phosphatase; ldhA, lactate dehydrogenase; pflB, pyruvate formate lyase; pdh, pyruvate dehydrogenase; poxB, pyruvate oxidase; pta, phosphotransacetylase; ackA, acetate kinase; adhE, alcohol/acetaldehyde dehydrogenase; maeAB, malic enzyme; mdh, malate dehydrogenase; fumABC, fumaraseABC; frd, fumarate reductase; sdh, succinate dehydrogenase; sucABCD, succinyl CoA synthase; icd, isocitrate dehydrogenase; acnAB, aconitate hydratase; gltA, citrate synthase

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