TY - JOUR AU - Zhao, Ying AU - Wang, Chang-Song AU - Li, Fei-Fei AU - Liu, Zhen-Ning AU - Zhao, Guang-Rong PY - 2016 DA - 2016/06/24 TI - Targeted optimization of central carbon metabolism for engineering succinate production in Escherichia coli JO - BMC Biotechnology SP - 52 VL - 16 IS - 1 AB - Succinate is a kind of industrially important C4 platform chemical for synthesis of high value added products. Due to the economical and environmental advantages, considerable efforts on metabolic engineering and synthetic biology have been invested for bio-based production of succinate. Precursor phosphoenolpyruvate (PEP) is consumed for transport and phosphorylation of glucose, and large amounts of byproducts are produced, which are the crucial obstacles preventing the improvement of succinate production. In this study, instead of deleting genes involved in the formation of lactate, acetate and formate, we optimized the central carbon metabolism by targeting at metabolic node PEP to improve succinate production and decrease accumulation of byproducts in engineered E. coli. SN - 1472-6750 UR - https://doi.org/10.1186/s12896-016-0284-7 DO - 10.1186/s12896-016-0284-7 ID - Zhao2016 ER -