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Table 2 Transfection efficiency of Phaseolus vulgaris leaf discs that were infiltrated by the SAAT method under different infiltration media

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Growth medium

Infiltration medium

Leaf discs*

Transient

Efficiency transfection (%)

Reference

LB

10 mM MgCl2

30

9

30

[55]

LB

10 mM MgCl2 and 5 mM MES-KOH

30

15

50

[56]

Winan’s AB

Winan’s AB

30

27

90

[54]

  1. *Ten leaf discs were used per biological replicate