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Table 2 Transfection efficiency of Phaseolus vulgaris leaf discs that were infiltrated by the SAAT method under different infiltration media

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Growth medium Infiltration medium Leaf discs* Transient Efficiency transfection (%) Reference
LB 10 mM MgCl2 30 9 30 [55]
LB 10 mM MgCl2 and 5 mM MES-KOH 30 15 50 [56]
Winan’s AB Winan’s AB 30 27 90 [54]
  1. *Ten leaf discs were used per biological replicate
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BMC Biotechnology

ISSN: 1472-6750

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