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Table 1 Conditions for protoplast isolation from various Phaseolus vulgaris tissues

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Plant sample Enzyme solution (ES) Vacuum infiltration Plasmolysis Digestion time Efficiency
Leaf ES-I 1.50 % (w/v) cellulase R10, 0.37 % (w/v) macerozyme R10 30 min N/A 4–5 h 3 × 105
cells g-1ml-1 FW
Flower petal ES-II 1.50 % (w/v) cellulase R10, 0.37 % (w/v) macerozyme R10, 30 U pectinase 30 min N/A 8–10 h 2 × 105
cells g-1ml-1 FW
Hypocotyl & root ES-III 2.0 % (w/v) cellulase R10, 0.3 % (w/v) macerozyme R10, 4.0 % (w/v) hemicellulase N/A 4 h 16–18 h 2 × 105
cells g-1ml-1 FW
Nodule ES-IV 1.0 % (w/v) cellulase R10, 0.3 % (w/v) macerozyme R10, 1.0 % (w/v) hemicellulase, 30 U of pectinase N/A 4 h 16–18 h 1 × 105
cells g-1ml-1 FW