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Fig. 8 | BMC Biotechnology

Fig. 8

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Fig. 8

Initial processing of Phaseolus vulgaris tissues for protoplast isolation. The plant material was sliced into 0.5–1 mm strips using a sterile scalpel blade and transferred to a Petri dish containing enzyme solution or medium for plasmolysis. a Leaf strips in enzyme solution. b Strips of wing and keel petals in enzyme solution. c Sliced nodules in plasmolysis solution

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