Skip to main content
Fig. 7 | BMC Biotechnology

Fig. 7

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Fig. 7

Schematic diagram of the gene structure and T-DNA harboring of SnRK1 and different the selectable marker genes. a Graphic representation of the gene structure of PvSnRK1 as predicted using the Phytozome v11 (https://phytozome.jgi.doe.gov/pz/portal.html). Green boxes illustrate exons, and gray boxes untranslated regions. The red line represents the non-conserved region of PvSnRK1 that is used to silence the target gene. b A schematic representation of the PvSnRK1-RNAi construct showing the sense and antisense region of PvSnRK1 at the LB. In the opposite orientation, the TdT expression cassette, which encodes a fluorescent selectable marker, is located toward the RB. This construct was carried by the pTdT-DC-PvSnRK1-RNAi binary vector. c The overexpression of the ORF of PvSnRK1 under the control of the constitutive 35S CaMV promoter at the LB. In the opposite orientation, the enhanced GFP expression cassette, which encodes a fluorescent selectable marker, is located towards the RB. This construct was carried by the pH7WG2D.1 binary vector. d pPZP-RCS binary vector constitutively expressing intron GUS. e pEarleyGate104 binary vector constitutively expressing yellow fluorescent protein (YFP). LB, left border; RB, right border; WRKY, hairpin loop; ORF, open reading frame

Back to article page