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Fig. 5 | BMC Biotechnology

Fig. 5

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Fig. 5

Transient gene expression by the improved SAAT method in Phaseolus vulgaris using the pPZP-RCS-GUS binary vector. a Ten-day-old plant that was grown in a growth chamber showing the second trifoliates (asterisk) suitable for the transient assay. Arrow- first trifoliate (from shoot apex). b The leaf discs in the vir-gene-induced Agrobacterium culture were first subjected to sonication and c later vacuum infiltrated in fresh Agrobacterium culture. d Co-cultivation of Agrobacterium-infected leaf discs on sterile filter paper moistened with MS basal medium. e The leaf discs that were transformed with empty vector were stained for the histochemical localization of β-glucuronidase (GUS) reporter activity. No blue-stained tissue appeared even after 24 h of incubation with the GUS assay buffer. f-g The leaf discs that were transformed with the pPZP-RCS-GUS vector were stained for the histochemical localization of β-glucuronidase (GUS) reporter activity. Blue staining appeared within 16 h of incubation

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BMC Biotechnology

ISSN: 1472-6750

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