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Fig. 1 | BMC Biotechnology

Fig. 1

From: Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis

Fig. 1

Phaseolus vulgaris plant material for protoplast isolation. a Ten-day-old wild type plant showing the suitable trifoliate size. b Fully bloomed flowers (~40 days after sowing) showing wing and keel petals. c Three-day-old decotyledoned germinated seed showing the appropriate stage of the hypocotyl. d The root tips of 3-day-old germinated seeds for root protoplast isolation. e The mature bean nodule 18–21 days after inoculation with Rhizobium tropici strain CIAT 899. Asterisks- the preferable portions of plant material for protoplast isolation

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