Fig. 7
TCDD detection by the sfGFP-AhR-based competitive ELISA. a Optimal concentration of the sfGFP-AhR for the assay was determined by indirect ELISA using serial logarithmic concentrations (nM) for incubation in TCDD-pre-coated wells. b Competitive ELISA was performed on serial logarithmic concentrations (ppt) of free TCDD, which were incubated with the rabbit-a-TCDD or the sfGFP-AhR (30 nM) before being transferred into the TCDD-pre-coated wells. The detection of bound sfGFP-AhR in both types of ELISA was performed using a rabbit anti-GFP, and both rabbit sera were detected with a goat anti-rabbit-HRP. The logarithmic fit equation and the accuracy (R2) are shown next to each curve