Fig. 6

Testing the interaction of the sfGFP-AhR with the TCDD by ELISA Indirect ELISA was performed using TCDD-pre-coated microplate. Anti-TCDD, sfGFP-AhR (100 nM) and sfGFP (100 nM) were added to the wells and the interaction was detected either by an anti-6 × His (a) or anti-GFP (b) antibodies. The graphical inset in each panel explains the principle of the detection method