Fig. 5

Purification and characterization of the sfGFP-AhR. a Diagram of the purification procedure using Ni⁺-NTA column installed on FPLC AKTA prime system. Continuous line represents the absorbance of the eluate, and the peaks of the flow-through sample and purified protein (sfGFP-AhR) are indicated. Dashed line represents conductivity of the eluate. b Detection of the purified sfGFP and sfGFP-AhR was done after SDS-PAGE separation either by blue staining or by immune blotting using anti-GFP or anti-6 × His antibodies. The protein molecular weight ladder is in the first lane (M). c Indirect ELISA for testing the purified sfGFP-AhR and sfGFP that were immobilized (10nM) on the microplate and detected by polyclonal and monoclonal anti-GFP or anti-6 × His tag antibodies (1:2000)