Fig. 4

Optimization of the in-vitro sfGFP-AhR refolding. a Several additives were added at their indicated optimal concentration to solubilize a pellet (0.1 mg in 1 ml) of sfGFP-AhR inclusion bodies (soluble fraction). Remaining insoluble precipitates were recovered by centrifugation and solubilized in 8 M urea (insoluble fraction). Serial molar concentrations of Lauroylsarcosine (b) and Arginine (c) used for solubilizing the pellet of sfGFP-AhR inclusion bodies. The RFU was measured (EX: 485/EM: 538) in soluble and insoluble fractions for each additive and for the different concentrations of the last two additives