Skip to main content
Fig. 2 | BMC Biotechnology

Fig. 2

From: Immuno-detection of dioxins using a recombinant protein of aryl hydrocarbon receptor (AhR) fused with sfGFP

Fig. 2

Construction of the pRSET-sfGFP-AhR plasmid. a PCR amplification was performed on the cDNA of HepG2 cells and the products were separated on a 1 % agarose gel; a fragment (265 bp) of actin gene as a control (lane 1), small domain from the AhR gene using internal primers (lane 2) and the full-length of LBD (lane 3). Expected sizes of the amplified bands are shown to the right. b 1 % agarose gel containing the pRSET-sfGFP-AhR (lanes 1&2) and pRSET-sfGFP (lanes 3&4); undigested (lanes 1&3) or digested with BamHI/EcoRI restriction enzymes (lanes 2&4). The extracted AhR insert is indicated with an arrow to the right

Back to article page