Fig. 1

Designing of the sfGFP-AhR fusion protein. a Schematic representation of the hAhR gene and the two recombinant proteins; sfGFP-AhR and sfGFP, used in this study. The different domains of the AhR gene are shown and the cloned LBD domain (231–428 aa) is indicated. The theoretical molecular size (kDa) and molecular weight (pMoles/μg) are shown to the right of each recombinant construct. Positions of the different elements; 6 × His tag, GGSSSG linker and glycosylation sites, are indicated using specific symbols ●, ♦ and *, respectively. b Cartoon representation of the modelled 3D structure of the sfGFP-AhR fusion, where TCDD binding cavity and the N-terminal 6 × His tag are shown. Structure simulation was predicted using Phyre2 server [47]