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Table 2 Dynamic range coverage

From: Evaluation of the External RNA Controls Consortium (ERCC) reference material using a modified Latin square design

Platform

Units

Subset

ERCC-00073

Minimum

Maximum

Range

Illumina

log2 signal

ERCC

5.81 ± 0.08

5.72 ± 0.07

13.88 ± 0.11

8.16 ± 0.13

Bead

BKGDa

5.35 ± 0.07

14.34 ± 0.08

8.99 ± 0.10

NIAID

log2 signal

ERCC

5.53 ± 0.02

5.53 ± 0.03

15.83 ± 0.38

10.30 ± 0.39

In-house

BKGD

5.20 ± 0.01

15.26 ± 0.27

10.06 ± 0.27

Agilent

log2 signal

ERCC

2.62 ± 0.14

2.57 ± 0.16

20.73 ± 0.18

18.16 ± 0.24

One-color

BKGD

2.41 ± 0.10

20.66 ± 0.06

18.25 ± 0.12

Agilent

log2 signal

ERCC

2.57 ± 0.06

2.40 ± 0.06

18.37 ± 0.10

15.98 ± 0.12

Two-color

BKGD

4.40 ± 0.15

20.00 ± 0.10

15.60 ± 0.19

Illumina

log2 FPKM

ERCC

Undetected

−4.98 ± 0.67

14.58 ± 0.27

19.56 ± 0.72

HiSeq

BKGD

−6.34 ± 0.40

18.27 ± 0.05

24.61 ± 0.40

LifeTech

log2 RPKMb

ERCC

Undetected

−3.26 ± 0.38

16.47 ± 0.34

19.73 ± 0.51

SOLiD

BKGD

−6.64 ± 0.00

17.30 ± 0.35

23.94 ± 0.35

  1. aAll transcripts measured in the total human liver RNA background
  2. bMinimum RPKM value reported is truncated at 0.01 for all replicates