|
Type-specific murine mAbs
|
|
mAbb
|
Epitope Specificity
|
Absorbance, 450 nm
|
| | |
DENV-3 E
|
DENV-2 E
|
DENV-1E
|
HBsAg
|
|
DENV1 E24
|
EDIII
|
0.03
|
0.02
|
1.23
|
0.03
|
|
DENV1 E29
|
EDIII
|
0.09
|
0.03
|
1.87
|
0.03
|
|
DENV1 E37
|
EDIII
|
0.03
|
0.02
|
0.71
|
0.04
|
|
DENV1 E103
|
EDIII, LR
|
0.06
|
0.06
|
3.48
|
0.02
|
|
DENV-2 3H5
|
EDIII, LR
|
0.06
|
3.46
|
0.14
|
0.06
|
|
DENV-3 8A1
|
EDIII, LR
|
1.35
|
0.02
|
0.08
|
0.03
|
|
DENV-4 E88
|
EDIII, LR
|
0.03
|
0.07
|
0.05
|
0.05
|
|
Cross-reactive murine & human mAbs
|
|
mAb
|
Epitope Specificity
|
Absorbance, 450 nm
|
| | |
DENV-3 E
|
DENV-2 E
|
DENV-1E
|
HBsAg
|
|
E17c
|
EDI/II
|
3.45
|
3.47
|
3.42
|
0.07
|
|
4G2d
|
Fusion loop
|
0.83
|
0.20
|
0.23
|
0.06
|
|
12C1d
|
EDIII, not LR
|
3.72
|
3.86
|
3.76
|
0.02
|
|
E77Com
|
EDIII, AS, LR
|
2.41
|
1.21
|
3.50
|
0.04
|
|
h-1M7d
|
Fusion loop
|
2.48
|
3.03
|
2.97
|
0.01
|
|
h-2 J20
|
EDIII
|
3.58
|
3.46
|
3.67
|
0.06
|
|
h-2 K2
|
prM
|
0.04
|
0.03
|
0.03
|
0.06
|
-
aDetermined by indirect ELISA using P. pastoris-produced purified DENV E VLPs as coating antigens. For comparison, corresponding data for DENV-2 E and DENV-3 E VLPs [14] are shown
-
bThe type-specific mAbs used are described in literature: DENV-1 mAbs E24, E29, E37 & E103 [17]; DENV-2 mAb 3H5 [22]; DENV-3 mAb 8A1 [23]; DENV-4 mAb E88 [24] . Cross-reactive mAbs: E17 [25]; 4G2 [22]; 12C1 [23]; h-1 M7 [27]; h-2 J20 [26] & h-2 K2 [28]; All mAb are murine, except the last 3 which are human (prefixed ‘h’)
-
cSub-complex-specific mAbs; E17 recognizes EDI/II of DENV-1 and DENV-3
-
dComplex-specific mAbs; 4G2 and h-1 M7 bind the fusion loop of all four DENV Es; 12C1 binds recombinant EDIII (outside the LR epitope) of all four DENV serotypes
