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Table 1 Analysis of antigenic integrity of DENV-1 E VLPsa

From: Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Type-specific murine mAbs
mAbb Epitope Specificity Absorbance, 450 nm
   DENV-3 E DENV-2 E DENV-1E HBsAg
DENV1 E24 EDIII 0.03 0.02 1.23 0.03
DENV1 E29 EDIII 0.09 0.03 1.87 0.03
DENV1 E37 EDIII 0.03 0.02 0.71 0.04
DENV1 E103 EDIII, LR 0.06 0.06 3.48 0.02
DENV-2 3H5 EDIII, LR 0.06 3.46 0.14 0.06
DENV-3 8A1 EDIII, LR 1.35 0.02 0.08 0.03
DENV-4 E88 EDIII, LR 0.03 0.07 0.05 0.05
Cross-reactive murine & human mAbs
mAb Epitope Specificity Absorbance, 450 nm
   DENV-3 E DENV-2 E DENV-1E HBsAg
E17c EDI/II 3.45 3.47 3.42 0.07
4G2d Fusion loop 0.83 0.20 0.23 0.06
12C1d EDIII, not LR 3.72 3.86 3.76 0.02
E77Com EDIII, AS, LR 2.41 1.21 3.50 0.04
h-1M7d Fusion loop 2.48 3.03 2.97 0.01
h-2 J20 EDIII 3.58 3.46 3.67 0.06
h-2 K2 prM 0.04 0.03 0.03 0.06
  1. aDetermined by indirect ELISA using P. pastoris-produced purified DENV E VLPs as coating antigens. For comparison, corresponding data for DENV-2 E and DENV-3 E VLPs [14] are shown
  2. bThe type-specific mAbs used are described in literature: DENV-1 mAbs E24, E29, E37 & E103 [17]; DENV-2 mAb 3H5 [22]; DENV-3 mAb 8A1 [23]; DENV-4 mAb E88 [24] . Cross-reactive mAbs: E17 [25]; 4G2 [22]; 12C1 [23]; h-1 M7 [27]; h-2 J20 [26] & h-2 K2 [28]; All mAb are murine, except the last 3 which are human (prefixed ‘h’)
  3. cSub-complex-specific mAbs; E17 recognizes EDI/II of DENV-1 and DENV-3
  4. dComplex-specific mAbs; 4G2 and h-1 M7 bind the fusion loop of all four DENV Es; 12C1 binds recombinant EDIII (outside the LR epitope) of all four DENV serotypes