Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Table 1 Analysis of antigenic integrity of DENV-1 E VLPs^a

Type-specific murine mAbs
mAb^b	Epitope Specificity	Absorbance, 450 nm
		DENV-3 E	DENV-2 E	DENV-1E	HBsAg
DENV1 E24	EDIII	0.03	0.02	1.23	0.03
DENV1 E29	EDIII	0.09	0.03	1.87	0.03
DENV1 E37	EDIII	0.03	0.02	0.71	0.04
DENV1 E103	EDIII, LR	0.06	0.06	3.48	0.02
DENV-2 3H5	EDIII, LR	0.06	3.46	0.14	0.06
DENV-3 8A1	EDIII, LR	1.35	0.02	0.08	0.03
DENV-4 E88	EDIII, LR	0.03	0.07	0.05	0.05
Cross-reactive murine & human mAbs
mAb	Epitope Specificity	Absorbance, 450 nm
		DENV-3 E	DENV-2 E	DENV-1E	HBsAg
E17^c	EDI/II	3.45	3.47	3.42	0.07
4G2^d	Fusion loop	0.83	0.20	0.23	0.06
12C1^d	EDIII, not LR	3.72	3.86	3.76	0.02
E77^Com	EDIII, AS, LR	2.41	1.21	3.50	0.04
h-1M7^d	Fusion loop	2.48	3.03	2.97	0.01
h-2 J20	EDIII	3.58	3.46	3.67	0.06
h-2 K2	prM	0.04	0.03	0.03	0.06

^aDetermined by indirect ELISA using P. pastoris-produced purified DENV E VLPs as coating antigens. For comparison, corresponding data for DENV-2 E and DENV-3 E VLPs [14] are shown
^bThe type-specific mAbs used are described in literature: DENV-1 mAbs E24, E29, E37 & E103 [17]; DENV-2 mAb 3H5 [22]; DENV-3 mAb 8A1 [23]; DENV-4 mAb E88 [24] . Cross-reactive mAbs: E17 [25]; 4G2 [22]; 12C1 [23]; h-1 M7 [27]; h-2 J20 [26] & h-2 K2 [28]; All mAb are murine, except the last 3 which are human (prefixed ‘h’)
^cSub-complex-specific mAbs; E17 recognizes EDI/II of DENV-1 and DENV-3
^dComplex-specific mAbs; 4G2 and h-1 M7 bind the fusion loop of all four DENV Es; 12C1 binds recombinant EDIII (outside the LR epitope) of all four DENV serotypes

ISSN: 1472-6750