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Fig. 6 | BMC Biotechnology

Fig. 6

From: Virus-like particles derived from Pichia pastoris-expressed dengue virus type 1 glycoprotein elicit homotypic virus-neutralizing envelope domain III-directed antibodies

Fig. 6

Analysis of the role of anti-EDIII antibodies in virus neutralization: DENV-1 E VLP antiserum was used without prior depletion (a) or treated as follows. It was incubated with immobilized MBP (b) or immobilized MBP-EDIII-1 (c) matrix. Residual antibody titers in these three DENV-1 E VLP antiserum treatment groups (a, b and c), were analyzed in indirect ELISA using purified recombinant MBP-EDIII-1, −2, −3, −4 protein (panel a) or DENV-1, −2, −3 E VLPs (panel b). Residual neutralizing antibody titers (FNT50) in the same three sera groups were estimated using the FACS assay (Panel c)

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