Fatty acid and metabolomic profiling approaches differentiate heterotrophic and mixotrophic culture conditions in a microalgal food supplement 'Euglena'

Zeng, Min; Hao, Wenlong; Zou, Yongdong; Shi, Mengliang; Jiang, Yongguang; Xiao, Peng; Lei, Anping; Hu, Zhangli; Zhang, Weiwen; Zhao, Liqing; Wang, Jiangxin

doi:10.1186/s12896-016-0279-4

Table 1 Fatty acid compostions (μg/mg dw)

From: Fatty acid and metabolomic profiling approaches differentiate heterotrophic and mixotrophic culture conditions in a microalgal food supplement 'Euglena'

	HL-4d	HL-7d	HL-9d	HD-4d	HD-7d	HD-9d
C12:0	1.54 ± 0.25^c	0.84 ± 0.05^d	0.78 ± 0.01^d	7.29 ± 0.48^a	4.56 ± 0.03^b	4.16 ± 0.17^b
C13:0	1.20 ± 0.37^c	0.71 ± 0.06^c	0.56 ± 0.04^c	7.11 ± 0.75^b	8.10 ± 0.07^a	7.73 ± 0.26^ab
C14:0	13.03 ± 2.93^d	8.04 ± 0.40^d	7.49 ± 0.32^d	43.18 ± 6.72^c	54.15 ± 3.11^b	66.11 ± 2.53^a
C15:0	1.17 ± 0.21^d	1.21 ± 0.06^d	1.06 ± 0.03^d	2.65 ± 0.19^c	5.20 ± 0.07^b	5.62 ± 0.20^a
C16:0	10.66 ± 0.63^c	11.84 ± 0.14^c	11.66 ± 0.62^c	8.90 ± 0.52^d	14.37 ± 0.20^b	21.65 ± 0.92^a
C16:1	2.89 ± 0.23^c	5.06 ± 0.27^a	5.45 ± 0.48^a	2.28 ± 0.20^d	3.32 ± 0.10^c	4.58 ± 0.03^b
C17:0	0.30 ± 0.07^d	0.33 ± 0.02^d	0.36 ± 0.03^d	0.56 ± 0.04^c	1.00 ± 0.04^b	1.73 ± 0.04^a
C16:2	3.79 ± 0.19^c	7.58 ± 0.07^b	10.56 ± 0.26^a	0.53 ± 0.03^e	0.87 ± 0.02^d	0.78 ± 0.07^d
C17:1	0.63 ± 0.12^b	1.07 ± 0.13^a	1.11 ± 0.17^a	0.72 ± 0.09^b	1.06 ± 0.04^a	1.22 ± 0.06^a
C16:3	10.96 ± 0.70^c	14.66 ± 0.59^b	16.05 ± 0.55^a	1.62 ± 0.07^e	2.67 ± 0.09^d	2.05 ± 0.17^de
C18:0	0.26 ± 0.06^cd	0.24 ± 0.03^cd	0.22 ± 0.00^d	0.31 ± 0.03^c	0.47 ± 0.02^c	0.85 ± 0.03^a
C16:4	6.06 ± 1.11^b	7.53 ± 0.59^a	8.01 ± 0.88^a	0.12 ± 0.02^c	0.17 ± 0.01^c	0.18 ± 0.01^c
C18:1	2.14 ± 0.12^d	3.39 ± 0.05^a	3.14 ± 0.17^b	1.29 ± 0.04^f	1.91 ± 0.02^e	2.53 ± 0.14^c
C18:2ω6	6.00 ± 0.33^c	9.98 ± 0.12^b	12.74 ± 0.39^a	1.14 ± 0.06^e	1.71 ± 0.06^d	1.72 ± 0.05^d
C18:3ω3	19.40 ± 1.64^b	23.54 ± 0.63^a	25.03 ± 1.44^a	1.71 ± 0.06^c	2.51 ± 0.11^c	1.88 ± 0.01^c
C20:2ω6	2.50 ± 0.20^c	2.72 ± 0.23^c	2.74 ± 0.14^c	2.66 ± 0.12^c	5.06 ± 0.19^b	5.76 ± 0.16^a
C20:3ω6	1.47 ± 0.11^c	1.09 ± 0.04^d	0.90 ± 0.04^e	1.41 ± 0.05^c	1.95 ± 0.08^a	1.67 ± 0.05^b
C20:4ω6	4.02 ± 0.33^d	5.05 ± 0.25^c	5.22 ± 0.26^c	3.86 ± 0.17^d	7.22 ± 0.25^b	8.17 ± 0.31^a
C20:4ω3	0.74 ± 0.05^c	1.02 ± 0.03^b	1.25 ± 0.09^a	0.53 ± 0.02^e	0.62 ± 0.03^d	0.64 ± 0.03^d
C20:5ω3	5.29 ± 0.25^d	6.07 ± 0.19^c	6.60 ± 0.28^ab	3.81 ± 0.10^e	6.30 ± 0.25^bc	6.85 ± 0.14^a
C24:0	0.46 ± 0.07^b	0.49 ± 0.06^b	0.42 ± 0.00^b	0.31 ± 0.04^c	0.43 ± 0.01^b	0.67 ± 0.02^a
C22:4ω6	0.46 ± 0.17^a	0.26 ± 0.03^a	0.17 ± 0.01^b	0.43 ± 0.02^a	0.47 ± 0.01^a	0.50 ± 0.02^b
C22:5ω6	6.66 ± 0.70^c	8.07 ± 0.34^b	8.67 ± 0.22^ab	4.97 ± 0.15^d	7.94 ± 0.26^b	9.12 ± 0.28^a
C22:5ω3	0.79 ± 0.28^a	0.47 ± 0.02^bc	0.33 ± 0.01^c	0.57 ± 0.03^abc	0.57 ± 0.02^abc	0.64 ± 0.03^ab
C22:6ω3	5.73 ± 0.53^a	5.95 ± 0.21^a	6.23 ± 0.26^a	3.33 ± 0.05^c	4.53 ± 0.22^b	5.01 ± 0.25^b
Sum	108.13 ± 5.87^c	127.21 ± 2.23^b	136.75 ± 4.59^b	101.30 ± 9.46^c	137.17 ± 1.50^b	161.82 ± 5.29^a

a, b, c, d, e, and f shows significant difference between each other

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