Table 1 Primers and synthesized oligonucleotides
Primer | Sequence(5’-3’) |
|---|---|
PP7-for | 5’-GGAATTCCATATGTCCAAAACCATCGTTC-3’ |
GFP-for | 5’-GCTCTAGAGCCGCCATGGTGAGCAAGGGCGAGGA-3’ |
GFP-r | 5’-CGGGATCC TAAGGGTTTCCATATAAACTCCTTATCATTACTTGTACAGCTCGTCCA-3’ |
PP7-r | 5’-CCGCTCGAGTTAACGGCCCAGCGGCAC-3’ |
2PP7-Protamine-for | 5’-GGGGTACC GTTAGCCGTCGCCGTCGCCGTCGCGGCGGTCGTCGCCGTCGCGAGGCAACCCGCACTCTG-3’ |
GFP-qRT-for | 5’-GCAGAAGAACGGCATCAA-3’ |
GFP-qRT-r | 5’-GGTGCTCAGGTAGTGGTTGT-3’ |
GAPDH-for | 5’-AAATGGTGAAGGTCGGTG-3’ |
GAPDH-r | 5’-GTGAGTGGAGTCATACTGGAAC-3’ |