Table 5 The oligonucleotides primers used in this work
Primer | Sequence (5’ → 3’) | Usage |
|---|---|---|
pr_PstI_F | AACTGCAGgtttatcggccgttgaatat | Amplify the par-rep gene |
pr_SalI_R | ACGCGTCGACggtgtttaaacagtgttacggt | |
0169_SacI_F | ATAGAGCTCtgaaagcggctggcgcgt | Amplify the 5’-UTR of GOX0169 promoter |
0169_XbaI_R | GCTCTAGAgcggaaggcgttataccctga | |
0264_SacI_F | ATAGAGCTCgttgcgcctgaatgagagg | Amplify the 5’-UTR of GOX0264 promoter |
0264_XbaI_R | GCTCTAGAttcggtctccctcgccgtaa | |
0452_SacI_F | ATAGAGCTCggcttcgtggtgaacgcc | Amplify the 5’-UTR of GOX0452 promoter |
0452_XbaI_R | GCTCTAGAtagtgacattccagcttggg | |
tufB_ SacI_F | ATAGAGCTCcgatggtaagaaatccactgc | Amplify the tufB promoter |
tufB_ XbaI_R | ATATCTAGAccaaaaccccgctccacc | |
GFP_XbaI_F | ATATCTAGAatggtgagcaagggc | Amplify the gfp reported gene |
GFP_HindIII_R | CCCAAGCTTctacttgtacagctc | |
SLDH_XbaI_F | GCTCTAGAggactttcagttctggaggctttcacca | Amplify the sldAB gene |
SLDH_EcoRI_R | CGGAATTCtcccacccgaaaaatggaaaaaacg | |
ADD_0169_F | acactgtttaaacaccgtgaaagcggctggcgc | Amplify the fuse fragments |
pQQ_Fuse0169_R | acatccgcgcggaaggcgttatac | |
pQQ_Fuse0169_F | ccttccgcgcggatgttcagg | |
tldD_FusepQQ_R | ccggctagaagatggcctctc | |
tldD_FusepQQ_F | gccatcttctagccggtctgttc | |
0169_FusetldD_R | ctttcaggatcttcttcatg | |
0169_FusetldD_F | tcgcgactgaaagcggctggc | |
ADD_0169_R | cggtacccggggatcctgcggaaggcgttatac | |
cyoBACD_XbaI_F | CGATTCTAGAactactgcaagccggaacgg | Amplify the terminal cytochrome bo 3 oxidase |
cyoBACD_SacI_R | ACTGGAGCTCaagggctggcaggatttctc | |
RT16S_F | gcggttgttacagtcagatg | - |
RT16S_R | gcctcagcgtcagtatcg | - |
RTsldh_F | atcatgccgaccaagcgtggc | - |
RTsldh_R | cgtcggcgaacgcggatcg | - |